David m bliesner validating chromatographic methods
A component with high affinity towards the stationary phase will take longer to travel through it than a component with low affinity towards the stationary phase and high affinity towards the mobile phase.
As a result of these differences in mobilities, sample components will become separated from each other as they travel through the stationary phase [2, 3].
Ion-pair chromatography allows the separation of complex mixtures of polar and ionic molecules.
The selectivity is determined by the mobile phase supplemented with a specific ion-pairing reagent which large ionic molecules are having a charge opposite to the analyte of interest, as well as a hydrophobic region to interact with the stationary phase.
In planar chromatography, the stationary phase is supported on a flat plate or in the pores of a paper.
Here the mobile phase moves through the stationary phase by capillary action or under the influence of gravity .
The degree to which a sample particle may adsorb to the stationary phase is determined by the relative polarity, or charge, that the particle may possess at a given time.
Partition chromatography in which separation is based mainly on differences between the solubilities of the components in the mobile and stationary phases. The stationary phase liquid is present as thin film on an inert solid support.
The mobile phase is then forced through the stationary phase.
The precision study was precision, robustness and repeatabilty. Hence the suggested RP-HPLC method can be used for routine analysis of Topiramate in API and Pharmaceutical dosage form.
INTRODUCTION Classification of Analytical Methods: Analytical methods can be separated into classical and instrumental methods.
Quantitative analysis by gravimetric or titrimetric techniques.
In Gravimetry, the amount of a substance is determined by the mass of product generated by a chemical reaction.